The natural triterpenoid miliaсin prevents methotrexate-induced oxidative stress and normalizes the expression of genes encoding the cytochrome P-450 2E1 isoform and glutathione reductase in the liver

Authors

  • O.V. Kalinina N.N. Blokhin Russian Cancer Research Center of the RAMS, 24, Kashirskoye hwy., Moscow, 115478, Russia
  • E.S. Kolotova The Orenburg State Medical Academy, 6, Soviet str., Orenburg, 460000, Russia
  • T.V. Panfilova N.N. Blokhin Russian Cancer Research Center of the RAMS, 24, Kashirskoye hwy., Moscow, 115478, Russia
  • A.A. Shtil The Orenburg State Medical Academy, 6, Soviet str., Orenburg, 460000, Russia
  • B.A. Frolov N.N. Blokhin Russian Cancer Research Center of the RAMS, 24, Kashirskoye hwy., Moscow, 115478, Russia

Keywords:

methotrexate, miliacin, liver toxicity, oxidative stress, gene regulation

Abstract

We studied the role of the natural triterpenoid miliacin (3-β-метокси-Δ18-oleanene) in the regulation of oxidative stress in the liver of (СВАхС57В16)F1 mice exposed to methotrexate. Miliacin attenuated methotrexate-induced lipid peroxidation as determined by an attenuation of thiobarbituric acid-reacting products in the liver. Furthermore, miliacin normalized the expression of genes encoding the 2e1 isoform of cytochrome Р-450 and glutathione reductase that were dramatically dysregulated by methotrexate. These results established the role of miliacin in modulation of redox genes, thereby providing evidence for a new mechanism of organ protection by this triterpenoid.

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Published

2013-01-21

Issue

Vol. 57 No. 1 (2013)

Section

Original research

How to Cite

[1]
2013. The natural triterpenoid miliaсin prevents methotrexate-induced oxidative stress and normalizes the expression of genes encoding the cytochrome P-450 2E1 isoform and glutathione reductase in the liver. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 57, 1 (Jan. 2013), 70–74.