Photoactivation of cytochrome с oxidase activity in liver mitochondria of Japanese quail by therapeutic doses of blue and red LED irradiation

Authors

  • Alexander Е. Dontsov N.M. Emanuel Institute of Biochemical Physics, Kosygina Str. 4, Moscow 119934
  • N. B. Sereznikova N.M. Emanuel Institute of Biochemical Physics, Kosygina Str. 4, Moscow 119934; M.V. Lomonosov Moscow State University, Leninskie Gory 1, Moscow 119991
  • L. S. Pogodina M.V. Lomonosov Moscow State University, Leninskie Gory 1, Moscow 119991
  • Т. S. Gurieva Institute of Medical-Biological Problems, Khoroshevskoe Shosse 76 A, Moscow 123007
  • P. P. Zak N.M. Emanuel Institute of Biochemical Physics, Kosygina Str. 4, Moscow 119934

DOI:

https://doi.org/10.25557/0031-2991.2018.03.25-30

Keywords:

mitochondria, cytochrome c oxidase activity, photobiomodulation, LED irradiation, blue light

Abstract

The aim was to study the effect of blue (450 nm) and red (630 nm) LED irradiation with different energy exposures on activity of mitochondrial cytochrome c oxidase. Methods. The study was performed on mitochondria isolated from the liver of Japanese quail Coturnix japonica. Cytochrome c oxidase activity was measured by the rate of oxidation of reduced tetramethyl-p-phenylenediamine in the presence of rotenone. Results. Irradiation of mitochondria with blue light at doses not exceeding 4 J/cm² caused approximately 5—15% stimulation of cytochrome c oxidase activity while doses higher than 5 J/cm² led to inhibition of this enzyme. Irradiation of mitochondria with red light also exerted a slight stimulating effect (10—20%) on cytochrome c oxidase activity compared to unirradiated samples. Conclusion. The study suggested that low-dose irradiation with blue light may produce a therapeutic effect similar to red light in photobiomodulation.

Downloads

Published

2018-10-05

Issue

Vol. 62 No. 3 (2018)

Section

Original research

How to Cite

[1]
2018. Photoactivation of cytochrome с oxidase activity in liver mitochondria of Japanese quail by therapeutic doses of blue and red LED irradiation. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 62, 3 (Oct. 2018), 25–30. DOI:https://doi.org/10.25557/0031-2991.2018.03.25-30.