Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions
DOI:
https://doi.org/10.25557/0031-2991.2018.04.219-222Keywords:
targeted proteomics, HPLC-MS/MS, SRMAbstract
This article introduces the reader to some methodological aspects of an experiment using HPLC-MS/MS with the registration of selective transitions in the targeted proteomics. Special attention is paid to the selection criterion of a peptide used as a surrogate analyte of the protein to be determined. The approaches to the selection of selective transitions for the selective detection of peptides are described in detail.Published
2018-11-21
Issue
Section
Methods
How to Cite
[1]
2018. Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 62, 4 (Nov. 2018), 219–222. DOI:https://doi.org/10.25557/0031-2991.2018.04.219-222.