Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions

Authors

  • Edward D. Virus The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. V. Ivanov The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • P. O. Bulgakova The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. A. Kubatiev The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315

DOI:

https://doi.org/10.25557/0031-2991.2018.04.219-222

Keywords:

targeted proteomics, HPLC-MS/MS, SRM

Abstract

This article introduces the reader to some methodological aspects of an experiment using HPLC-MS/MS with the registration of selective transitions in the targeted proteomics. Special attention is paid to the selection criterion of a peptide used as a surrogate analyte of the protein to be determined. The approaches to the selection of selective transitions for the selective detection of peptides are described in detail.

Published

2018-11-21

Issue

Vol. 62 No. 4 (2018)

Section

Methods

How to Cite

[1]
2018. Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 62, 4 (Nov. 2018), 219–222. DOI:https://doi.org/10.25557/0031-2991.2018.04.219-222.

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