Effect of damage integrity rat brain synaptic membranes on the functional activity GABAₐ-receptor/Cl⁻-ionophore complex in the CNC

Authors

  • I.G. Rebrov Institute of general pathology and pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia
  • M.V. Kalinina Institute of general pathology and pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia

Keywords:

GABAₐ-receptor, synaptoneurosomes, isotope ³⁶Cl⁻, Са⁺², Mg⁺²

Abstract

Functional activity of the GABAA-receptor/Cl-ionophore complex was investigated the muscimol-stimulated entry of the radioactive isotope 36Cl− in synaptoneurosomes in changing the structure and permeability of neuronal membranes. Integrity of the membranes was damaged by removal of Ca+2 and Mg+2 from the incubation medium and by the method of freezing-thawing synaptoneurosomes. In both cases, an increase in basal 36Cl− entry into synaptoneurosomes, indicating increased nonspecific permeability of neuronal membranes, and decreased activity the GABAA-receptor/Cl−- ionophore complex. The conclusion about the relationship of processes damage neuronal membranes and reducing the inhibitory processes in the epileptic focus.

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Published

2013-01-21

Issue

Vol. 57 No. 1 (2013)

Section

Original research

How to Cite

[1]
2013. Effect of damage integrity rat brain synaptic membranes on the functional activity GABAₐ-receptor/Cl⁻-ionophore complex in the CNC. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 57, 1 (Jan. 2013), 37–40.