The effect of polylactide wound dressings with pro- and antioxidant components on thiol homeostasis and the activity of superoxide dismutase and catalase in rats with full-thickness skin defects

Abstract

Introduction. Free radical processes are an integral part of wound healing, on the one hand having an altering effect on one’s own tissues, and on the other hand being important regulators of reparative processes. The use of targeted delivery of pro-oxidants and antioxidants in wound treatment opens up new prospects for improving outcomes of regeneration. In this regard, the aim of this study was to investigate the effect of microchamber polylactide wound dressings providing targeted delivery of pro- and antioxidant components on the state of enzymatic and non-enzymatic components of antioxidant protection in rats with full-thickness skin surgical wounds. Methodology. The study was carried out on 81 white male rats, divided into five groups: control (n=9), comparison (n=18), three experimental (n=18 each). In animals of the comparison and experimental groups a model of a skin wound 10x10 mm was surgically created. In animals of experimental group No. 1 a polylactide microchamber wound dressings without active components were applied to the formed skin defect. Rats of experimental groups No. 2 and 3 received a similar dressings, the microchambers of which were loaded with tannic acid and sodium percarbonate, respectively. The activity of superoxide dismutase and catalase, as well as serum concentration of total thiol groups, were assessed. Results and conclusions. It was revealed that skin damage cause changes of enzymatic and non-enzymatic components of antioxidant protection. The use of a polylactide microchamber wound dressings loaded with tannic acid neutralizes changes in the parameters of antioxidant protection, preventing a decrease in the concentration of thiol groups and an increase in the activity of superoxide dismutase and catalase. The use of a polylactide microchamber wound dressings loaded with sodium percarbonate in the area of the wound defect provides reducing of the activity of superoxide dismutase and restoring the concentration of thiol groups in the blood serum at the later stages of the reparative process, but has no significant effect on the activity of catalase. Thus, loading pro- and antioxidant components into a microchamber polylactide dressings allows to correct changes in thiol status, superoxide dismutase and catalase activity in rats with full-thickness skin defects.