Registration of the protein in the serum with a field-effect nanotransistor biosensor

Authors

  • Yu. D. Ivanov Institute of Biomedical Chemistry, Moscow, Russia; TSAMAX Ltd., Moscow, Russia
  • К. А. Malsagova Institute of Biomedical Chemistry, Moscow, Russia
  • Т. О. Pleshakova Institute of Biomedical Chemistry, Moscow, Russia
  • I. D. Shumov Institute of Biomedical Chemistry, Moscow, Russia
  • A. L. Kaysheva Institute of Biomedical Chemistry, Moscow, Russia
  • V. P. Popov Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • O. V. Naumova Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • B. I. Fomin Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • D. A. Nasimov Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • A. V. Latyshev Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • A. L. Aseev Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • V. Yu. Tatur TSAMAX Ltd., Moscow, Russia
  • N. D. Ivanova Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia
  • G. M. Konovalova Sochi State University, Sochi, Russia
  • A. I. Archakov Institute of Biomedical Chemistry, Moscow, Russia

Keywords:

the field transistor; detection of albumen; biologysensor

Abstract

A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of “silicon-on-insulator” structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein. Using the 3 um-NW enabled to obtain stable source-drain characteristics and to register D-NFATc1 in serum at concentration of 2.5×10-14 M in the mode of drain-source current vs. gate voltage characteristics measurements. Data collection in the mode of drain-source current vs. gate voltage characteristics measurements was carried out with the use of high-speed data collection system running TURBO NBS software.

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Published

2016-03-22

Issue

Vol. 60 No. 1 (2016)

Section

Methods

How to Cite

[1]
2016. Registration of the protein in the serum with a field-effect nanotransistor biosensor. Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 60, 1 (Mar. 2016), 94–98.

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