Content of neutrophils carriying adhesion molecules in patients with psoriasis

Abstract

Studying regulation of neutrophil migration to the skin in psoriasis is of both theoretical and practical interest since high-intensity epidermal infiltration by neutrophils is associated with more severe disease. The process of neutrophil transmigration through the microvascular endothelium is known to be regulated by adhesion molecules. The aim of the study was to measure the content of neutrophils expressing adhesion molecules based on neutrophil nucleus segmentation. Methods. Specific gravity of adhesion molecules expressed on neutrophils and segmentation of neutrophil nuclei were determined in venous blood. The study included 82 patients (39 women and 43 men aged 20-60) with vulgar and exudative psoriasis at progressive and stationary stages. The control group consisted of 50 healthy individuals (28 women and 22 men). Results. Progressive and stationary stages of psoriasis were associated with a decreased number of neutrophils carrying adhesion molecules, except for the increased number of neutrophils with L-selectin molecules. Analysis of neutrophil segmentation in patients with psoriasis showed a higher specific gravity of neutrophils with 2 and 3 nuclear lobes («segments»); the content of neutrophils with 4 and 5 nuclear lobes was significantly lower. Conclusion. The results indicate increased activity of neutrophil migration from blood into tissue at progressive and stationary stages of psoriasis.