Concentrations of glycoxidation products in mixed saliva correlates with age

Abstract

Introduction. Oxidative stress is believed to be one of the mechanisms of aging, and oxidative stress products can be considered potential biomarkers of aging. The aim of this study was to evaluate age-related changes in concentrations of malondialdehyde and advanced glycation end products in mixed saliva from healthy individuals of different age groups and to assess their potential as biomarkers of aging. Methods. Mixed saliva samples were obtained from healthy children (n=22), young adults (n=26), middle-aged adults (n=20) and elderly people (n=20). Malondialdehyde (ELISA kit for Malondialdehyde, Cloud-Clone, China) and advanced glycation end product (ELISA kits for Advanced Glycation End Products, Cloud Clone, China) were measured by solid-phase enzyme immunoassays. To account for the dilution and viscosity parameters of the mixed saliva, the results were standardized by total protein concentration of the samples using the Mindray kit (China). Results. The concentration of malondialdehyde did not differ between the age groups. The concentration of advanced glycation end products increased in the elderly group, both before and after standardization. We found that the immediate and standardized concentrations of advanced glycation products were correlated with the age. The simple linear regression method was used for characterizing the significant linear dependence on the chronological age. Conclusion. The revealed age dependence of the concentration of advanced glycation end products in mixed saliva allows considering these compounds as potential biomarkers of aging.